Golden Gate Reaction Builder

Default target backbone (fmol)Minimum pipette volume (µL)These mirror the robot protocol's runtime parameters and only affect the live preview below — set the real values in the Opentrons App when you run the protocol.

Predefine named locations. Saved in this browser.

Reaction IDDestination wellTotal volume (µL)

Backbone

1×—

Missing conc/size/molar ratio

Override target backbone amount for this reaction (using default: 50 fmol)

Inserts

—

Missing conc/size/molar ratio

Reagents

Missing volume

Water

Waiting on other components

Computed total: 0 µL / 20 µL target

gga.trevfox

Backbone

Inserts

Reagents

Water